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. 2018 Sep 25;11:1999–2009. doi: 10.2147/JPR.S168715

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© 2018 Liang et al. This work is published and licensed by Dove Medical Press Limited

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Distribution of microvessels in endometriotic lesions with/without PNI.

Notes: Double immunofluorescence was performed to present microvessels and nerve fibers simultaneously. Microvessels were identified by antibody against CD31 (a specific marker of endothelial cells), which was red-stained, whereas nerve fibers were identified by antibody against PGP 9.5, which was green-stained. Nucleus was blue-stained with DAPI. More microvessels were found in endometriotic lesions of the PNI (+) group (A), compared to that of the PNI (−) group (B). Exact quantization of the MVD was acquired in both USL-DIE and RVS-DIE lesions. Further comparison analysis showed similar results: there was an increased MVD in endometriotic lesions of the PNI (+) group than that of the PNI (−) group (C and D).

Abbreviations: PNI, perineural invasion; MVD, microvessel density; USL-DIE, endometriosis infiltrating the uterosacral ligament; RVS-DIE, endometriosis involving the rectovaginal septum.