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(A) Chondrocytes were transfected with si-PVT1, prior to exposure to IL-1β for 24 h. Then, the concentration of PGE2 in supernatants was detected by ELISA. (B) The production of NO was analyzed by a NO detection kit. (C–E) The inflammatory cytokine levels of IL-6 (C), IL-8 (D) and TNF-α (E) in culture medium were also measured by ELISA. *P<0.05 vs. control group. #P<0.05 vs. IL-1β-treated group.
