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. 2018 Sep 24;9:567. doi: 10.3389/fendo.2018.00567

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Copyright © 2018 Chen, Tang, Wang, He, Guo, Liu, Liu and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Steroidogenesis in esr1 mutant female zebrafish. (A) FSHb and LHb levels at 90 dpf and 180 dpf were measured via Western bolt analysis. GAPDH was used as the reference. (B,C) Whole-body estradiol (B) and 11-KT (C) levels were measured via ELISA. (D–K) The relative expression levels of steroidogenesis pathway genes in the ovaries of wild-type and esr1 mutant zebrafish at 90 dpf and 180 dpf. The mRNA levels were normalized to ef1a. Average mRNA levels in the ovaries of wild-type were defined as 1.0. (^*P < 0.05; ^**P < 0.01; ^***P < 0.001; Mean ± SEM).