Figure 2.

Gene editing of pgrmc1 in zebrafish. (A) Comparison of mutant genomic DNA sequences (small insertions or deletions) in pgrmc1^−/− to those in wildtype (wt). Modified sequence region is highlighted in red, whereas adjacent un-modified sequences are highlighted in yellow. (B) Schematic drawings show wt Pgrmc1 protein and predicated truncated proteins from pgrmc1^−/− lacking heme binding domains. (C) Gel images of PCR products using a pgrmc1 wildtype specific primer (a), or a pgrmc1 mutant specific primer (b) to distinguish Pgrmc1 mutants from wildtype fish. (D) Western analysis confirmed loss of Pgrmc1 protein in pgrmc1^−/− using Stage IVa follicles and a zebrafish Pgrmc1 specific antibody.