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. 2018 Sep 24;9:560. doi: 10.3389/fendo.2018.00560

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Copyright © 2018 Wu, Thomas and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of ovarian size, morphology and spawning frequency between pgrmc1^−/− and wildtype. (A) Gonadosomatic index (GSI) of wildtype (wt) and pgrmc1^−/−. (B) HE staining of a representative ovarian section from a pgrmc1^−/− female showed well-formed different stages of oocytes with a significantly higher number of Stage I follicles compared to wildtype (wt). Scale bars: 500 μm. (C) A higher number of Stage I follicles but a lower number of late stage follicles were observed in pgrmc1^−/− compared to wt. (D) Mutant females spawned with less frequency than wt. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.