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. 2018 Aug 20;12(10):1706–1717. doi: 10.1002/1878-0261.12365

Figure 4.

Figure 4

CIP2A sustains survival of colon cancer cells through ATF6 under both of basal and ER stress conditions. HCT15 and SW480 cells were transfected with shRNA against luciferase or CIP2A for 48 h, and whole‐cell extracts were subjected to western blot analysis using antibodies specific for CIP2A and β‐actin (A) and to MTT assays (B). HCT15 cells were co‐transfected with ATF6 (1–373)‐expressing or control plasmid and shRNA against CIP2A (shCIP2A) or luciferase (shLuc) for 48 h. The transfected cells were further analyzed by western blotting using anti‐ATF6, anti‐CIP2A, and anti‐β‐actin antibodies (C) and by MTT assays (D). The means ± SEM of three independent experiments performed in triplicate are shown. Statistical analysis was carried out using Student's t‐test. *P < 0.05, ***P < 0.001.