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. 2018 Aug 20;9(10):1262–1270. doi: 10.1111/1759-7714.12830

Figure 2.

Figure 2

GLI1 was a potential target gene of miR‐873. (a) miR‐873 messenger RNA (mRNA) expression was evaluated by quantitative real‐time (qRT)‐PCR. qRT‐PCR and Western blot assays were performed to assess GLI1 (b) mRNA and (c) protein levels. *P < 0.01 versus control; **P < 0.01 versus mock. (d) The binding site of GLI1 to miR‐873 was predicted. The GLI1 3’ untranslated region (UTR)‐mutant (mut) is presented. (e) The 3’UTR of GLI1 with affinity for miR‐873 and a mutant reporter were cloned to the downstream of firefly luciferase of psiCHECK‐2 vector. miR‐873 was transfected into HEK293T cells. Luciferase activity was examined. *P < 0.01 versus GLI1 3’UTR; **P < 0.01 miR‐873 + GLI1–3’UTR + mut. NS, not significant.