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. 2018 Sep 24;9:402. doi: 10.3389/fgene.2018.00402

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Copyright © 2018 O’Brien, Weston, Sheth, Bradley, Bigbee, Pandey, Williams, Wolstenholme and Miles.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ethanol behavioral sensitization in male DBA2/J mice. (A) Experimental protocol and timeline for induction of behavioral sensitization. (B) Repeated ethanol exposure induced behavioral sensitization as measured by locomotor activity on day 14 (EE) as compared to acute ethanol administered on day 3 (EE) and day 14 (SE). (C) Experimental groupings used for RNA sequencing and bioinformatic analysis were derived from ethanol treatment type and specific cellular fraction. (#p < 0.001 compared to SS within same day, $p < 0.001 compared to SE within same day, ^∗p < 0.001 compared to same treatment on day 3, repeated measures two-way ANOVA with Tukey’s post hoc analysis).