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. 2018 Aug 13;37(19):e99021. doi: 10.15252/embj.201899021

Figure 2. Displacement of Pol alpha from the replisome leads to a loss of gene silencing at sub‐telomeric or mating‐type loci.

Figure 2

  • A–C
    Tetrad analysis of meiotic progeny of the indicated diploids (heterozygous for sub‐telomeric ADE2), processed as described in Materials and Methods. Sectored colonies represent epigenetic variation in the expression of the ADE2 marker at the sub‐telomeric location. White colonies indicate expression of ADE2 in all cells, and red colonies lack the ADE2 marker (as denoted in the right‐hand panels). We examined a total of 19 mcm2‐3A VR::ADE2‐TEL colonies and 11 pol1‐4A VR::ADE2‐TEL colonies.
  • D–F
    Equivalent analysis of the meiotic progeny of diploid cells that were heterozygous for an insertion of the ADE2 marker at HMR locus on chromosome 3 (denoted HMR::2EDA since the orientation of the ADE2 marker was such that the promoter was distal to the HMR‐E silencer element). We examined a total of 19 mcm2‐3A HMR::2EDA colonies and 12 pol1‐4A HMR::2EDA colonies.