Figure EV2. Replisome‐tethered histone‐binding activity of Mcm2 and Pol alpha is required for gene silencing at the HMR mating‐type locus.

• A–E
  Tetrad analysis of meiotic progeny of the indicated diploids (heterozygous for insertion of the ADE2 marker gene at the HMR locus on chromosome 3), processed as described in Materials and Methods. The promoter of the ADE2 marker was positioned proximal to the HMR‐E silencer element (denoted HMR::ADE2), and this corresponded to weaker silencing of ADE2 compared to the HMR::2EDA strains in Fig 2D–F. We examined a total of 16 mcm2‐3A HMR::ADE2 colonies, 16 pol1‐4A HMR::ADE2 colonies, 15 pol1‐2A2 HMR::ADE2 colonies and 16 pol1‐6A HMR::ADE2 colonies.