Table 1.
Mass spectrometry analysis of proteins co‐purifying with Pol1NT, Mcm2NT or Mcm4NT, +/−DNase treatment to release histone complexes from chromatin
| Identified protein | MS analysis of IPs from G2‐M cell extracts (spectral counts) | ||||
|---|---|---|---|---|---|
| Pol1 (1–351) +DNase | Pol1 (1–351) −DNase | Mcm2 (1–200) +DNase | Mcm2 (1–200) −DNase | Mcm4 (1–186) +DNase | |
| Pol1NT | 1,570 | 854 | 7 | 0 | 0 |
| Mcm2 | 436 | 0 | 258 | 218 | 6 |
| Mcm4 | 38 | 11 | 14 | 14 | 681 |
| Ctf4 | 938 | 564 | 0 | 0 | 0 |
| Tel1 | 407 | 229 | 18 | 0 | 0 |
| Histone H2A | 95 | 0 | 46 | 0 | 16 |
| Histone H2B | 441 | 3 | 140 | 3 | 33 |
| Histone H3 | 275 | 2 | 178 | 5 | 13 |
| Histone H4 | 287 | 4 | 275 | 4 | 28 |
| Spt16 | 2,255 | 53 | 1,558 | 5 | 201 |
| Pob3 | 812 | 8 | 486 | 0 | 46 |
| Tra1 | 600 | 32 | 1,105 | 44 | 86 |
| Spt5 | 396 | 22 | 38 | 3 | 26 |
The indicated protein fragments were expressed in G2‐M‐arrested cells and then isolated by immunoprecipitation on IgG‐coated magnetic beads. The resultant material was resolved in a 4–12% gradient gel, which was then stained with colloidal Coomassie blue, before each lane was cut into 40 bands for mass spectrometry analysis. The table summarizes the spectral counts that were detected for each factor that specifically co‐purified with the indicated fragment.