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. 2018 Sep 24;11:347. doi: 10.3389/fnmol.2018.00347

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Copyright © 2018 Benito-Muñoz, Perona, Abia, dos Santos, Núñez, Aragón and López-Corcuera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GlyT1 and GlyT2 alignment and mutant expression. (A) Alignment of the amino acid sequences of TM2 and TM10 of rat NSS transporters using the ClustalW program. (B,C) Structural alignment of modeled GlyT1 and GlyT2 showing GlyT2 Glu250 and Glu650 and GlyT1 Glu89 and Met475 in stick representation. (D,E) Surface expression of GlyT2 (D) and GlyT1 (E) mutants assessed by surface biotinylation. T, total transporter; B, biotinylated transporter; TfRc, transferrin receptor as loading control.