Table 1.

Kinetic parameters of glycine transport by GlyT2 and GlyT1 mutants in transfected COS7 (150 mM NaCl).

Mutant	Vmax (nmol/mg/10 min)	Km (μM)	Vmax/Km
wtGlyT2	32.5 ± 8.7	133.5 ± 25.7	0.24 ± 0.04
E250D	14.2 ± 3.9	92.6 ± 6.7	0.16 ± 0.04
E650D	11.8 ± 4.3	66.7 ± 25.9	0.18 ± 0.03
E650M	17.7 ± 7.2	102.4 ± 33.7	0.15 ± 0.03
E250A/E650M	35.8 ± 19.1	177.2 ± 103.4	0.21 ± 0.01
wtGlyT1	114.6 ± 11.1	199.9 ± 35.0	0.62 ± 0.17
M475D	91.3 ± 0.5	630.0 ± 163.7	0.17 ± 0.04
M475E	45.6 ± 17.5	542.6 ± 168.9	0.12 ± 0.05