Table 1.

Number of artesunate pressure cycles (APC) used to select for single-dose resistance in individual mice

Dose of Artesunate (mg/kg)	2.4	3.3	4	15	30	60	120	240
Number of APC required to reach resistance	3	2	2, 3, 1, 2, 1	3, 9, 6, 2	1, 2, 4, 5, 3, 4, 2	5, 1	3, 3, 4, 2, 3, 3, 2, 2, 2, 2, 2	4, 1, 7, 1, 2, 1, 2, 1, 1, 1, 1
Number of mice with resistance/total attempted	1/4	1/3	5/5	4/5	7/8	2/2	11/11	11/15

The number of artesunate pressure cycles (APC) after which resistance was seen to a given dose in individual mice is tabulated. The proportion of mice with parasites that evolved resistance can be seen to increase as the resistance strengthened, and as ‘fitter’ parasites were selected out by successive sub-inoculations, until the maximum dose was reached. A full account of the selection process and evolution of ART-R for the single and 2-day regimens can be found in the supplementary information (Additional files 1, 2, 5 and 8)