Figure 1.

Generation of M-LPH-KO HepG2 cells using the CRISPR/Cas9 system. (a) The sgRNA was designed to target 20 pairs of bases in exon 1 of the M-LPH gene. (b) Sequences of the WT and two knockout (KO) clones. Clone 1 had one allele with a single T insertion while the other had a single G insertion. Clone 2 was homozygous for a single T insertion. (c) Results of sequence analysis of the wild-type (WT) and two established clones. (d) Western blot analysis of M-LPH was performed using cell extracts obtained from the WT and established clones. β-Actin was used as a loading control.