Skip to main content
. 2018 Sep 17;2018:6956414. doi: 10.1155/2018/6956414

Figure 2.

Figure 2

Confocal immunofluorescence and Western blotting analyses of M-LPH-WT and -KO HepG2 cells using specific antibodies. (a) In M-LPH-WT cells, the anti-M-LPH antibody displayed faint nuclear and cytosolic staining, and a small number of punctate staining foci were observed within the cytoplasm. All of these foci were colocalized with a subset of mitochondria. DAPI and MitoTracker were used for nuclear and mitochondrial staining, respectively. Scale bar: 10 μm. (b) Subcellular fractionation/immunoblotting analysis of M-LPH-WT cells using specific antibodies against M-LPH. CE: cytosolic extract; ME: membrane extract; NE: nuclear soluble extract. Loading controls for CE, ME, and NE were Hsp90AA1, VDAC, and HDAC1, respectively. (c) M-LPH-KO resulted in a significant increase of γ-H2AX formation. (d) Western blot analysis of chromatin-bound extracts from MLPH-WT and -KO HepG2 cells. HIST1H1C was used as a loading control for nuclear protein. (e, f) Immunofluorescence detection showed that the protein level of TFAM was downregulated by M-LPH-KO (e), whereas that of TWNK was upregulated (f).