| 36.5 | μl ddH2O |
| 10 μl | 5X HF buffer |
| 1.0 μl | dNTPs, 10 mM |
| 0.5 μl | 100 μM Forward Primer |
| 0.5 μl | 100 μM Reverse Primer |
| 1.0 μl | template DNA |
| 0.5 μl | Phusion® polymerase |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.