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. 2018 Sep 28;6:e5679. doi: 10.7717/peerj.5679

Figure 1. Curing of ScVL1(ScVLA) by expression of DhVcp1.

Figure 1

Crude total RNA preparations from pYes transformants grown for many generations in galactose minimal medium and from pG3 transformants grown for many generations in glucose minimal medium were prepared by phenol extraction of whole cells. (A) is crude RNA run on a 1.4% agarose gel. (B) is RTPCR products from these same samples using primers from the RdRp region of L1 on a 1.4% agarose gel. (C) is RTPCR products from the same samples using primers from S. cerevisiae RPS11B, bracketing its intron, again run on a 1.4% agarose gel. Markers were the GeneRuler 1 kb DNA ladder (Thermo Fisher Scientific, Waltham, MA, USA). This is a composite of the three gels run with the same markers.