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. 2018 Sep 28;6:e5679. doi: 10.7717/peerj.5679

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©2018 Warner et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Western of pYes derived his tagged proteins and elaborated on a 7.5% SDS-PAGE, transferred to PVDF membrane, probed with anti-his tag antibody conjugated to horse radish peroxidase (HRP), developed with an HRP substrate, and exposed to film. Lane 1 is crude protein from pYEScp1 transformed cells grown in glucose; lane 2 is crude protein from pYEScp1 transformed cells grown in galactose; lane 3 is pYEScp1 derived protein (from DhVcp1) purified using nickel affinity magnetic beads; and lane 4 is purified pYEScap1 derived protein (from ScVL1). Markers are his-tagged proteins run in an adjacent lane.