Fig. 1.

RanGTP-induced MT self-organization in Xenopus egg extracts. A, Representative fluorescence images of RanGTP induced MT assemblies in egg extract upon incubation with RanGTP. Scale bar = 20 μm. B, Quantification of the different types of MT assemblies in 1 μl of egg extract at different times of incubation as indicated. Red bars, MT asters with long MTs; Orange bars, MT asters with short MTs; Blue bars, minispindles; Brown bars, aggregates of MT assemblies. Data obtained from four independent experiments. C, Colloidal blue stained SDS-PAGE of the MT pellets obtained at different time of incubation of egg extracts with RanGTP (as indicated). Molecular weight markers are indicated on the left in kDa. D, Schematic representation of the conversion and filtering process of the Xenopus proteins identified by mass spectrometry to obtain the final RanMT proteome of human proteins.