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. 2018 Jul 6;17(10):2005–2017. doi: 10.1074/mcp.RA118.000882

Fig. 1.

Fig. 1.

Lowering cellular NAD levels decreases cell growth rate. A, Western blotting confirmed CD38 overexpression in A549 cells. B, qPCR analysis confirmed overexpressed CD38 mRNA levels in A549 cells (n = 3). C, Western blotting confirmed CD38 overexpression in HepG2 cells. D, E, Cellular NAD and NADH levels in CD38(−) and CD38(+) A549 cells (n = 3). F, Growth curves of CD38(−) and CD38(+) A549 cells determined by CCK-8 assay (n = 3). G, Tumor samples harvested from mice 37 days after injection (n = 3). Tumor volumes (mm3) were measured using digital calipers and calculated using the formula: π/6 × length (mm) × width2 (mm). H, Growth curves of untreated- and 10 nm FK866-treated A549 cells determined by CCK-8 assay (n = 3). Data were analyzed using Student's t test. *p < 0.05; **p < 0.01; ***p < 0.001. *p < 0.05 was considered statistically significant. All values represent mean from at least three biological replicates ± S.E.