Table I. The top 10 differentially maternally-expressed proteins in the 2-cell stage of early embryos.
| Uniprot accession no. | Protein name | Gene symbol | MW [kDa] | Calc. pI | Fold changea | Regulationa | Subcellular location |
|---|---|---|---|---|---|---|---|
| A7E320 | E3 ubiquitin-protein ligase UHRF1 | UHRF1 | 88.28 | 8.00 | 1.40/1.38 | Up | Nucleus |
| F1MRC2 | Myosin-2 OS | MYH2 | 223.19 | 5.80 | 1.28/1.50 | Up | Cytoplasm |
| Q3SYU9 | Major vault protein | MVP | 98.86 | 5.60 | 1.28/1.22 | Up | Cytosol |
| P63097 | Guanine nucleotide-binding protein G(i) subunit alpha-1 | GNAI1 | 40.34 | 5.97 | 1.32/1.18 | Up | Plasma membrane |
| A7MBC2 | Pyridoxal-dependent decarboxylase domain-containing protein 1 | PDXDC1 | 86.56 | 5.39 | 1.23/1.25 | Up | Cytosol |
| Q3SYT9 | Poly(RC) binding protein 2 | PCBP2 | 36.78 | 7.06 | 0.71/0.82 | Down | Cytosol |
| Q3SZ86 | 28S ribosomal protein S26, mitochondrial | MRPS26 | 23.76 | 9.83 | 0.75/0.78 | Down | Mitochondrion |
| E1BHF2 | Katanin p60 ATPase-containing subunit A-like 1 | KATNAL1 | 55.35 | 6.74 | 0.76/0.84 | Down | Cytoplasm, cytoskeleton |
| G3X705 | 28S ribosomal protein S6, mitochondrial | MRPS6 | 13.77 | 9.26 | 0.80/0.81 | Down | Mitochondrion |
| A5D7N3 | Transmembrane protein 11, mitochondrial | TMEM11 | 21.46 | 7.33 | 0.80/0.83 | Down | Mitochondrion inner membrane |
aNote: The quantitative proteomic experiments were conducted twice. The fold change and regulation data were all compared with the data from MII stage. p value <0.05 and fold change >1.2 were considered significant.