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. 2018 Aug 15;293(39):14989–15001. doi: 10.1074/jbc.RA118.005089

Figure 1.

Figure 1.

Effect of E3 expression on TLR-stimulated cytokine induction in human monocytes. A, THP-1 cells were transduced with retroviruses encoding HA-tagged E3 or empty vector (EV). After cell selection, lysates were generated and immunoblotted for HA-E3 and β-actin, alongside a lysate from HEK293T cells transiently expressing E3. B–E, E3- or EV-expressing THP-1 cells were stimulated with 2 μg/ml of Pam3CSK4 (PAM3), 2.5 μg/ml of CLO75, 100 ng/ml of LPS or medium alone. The supernatants were removed 24 h later and TNFα (B), IL-6 (C), IP10 (D), and RANTES (E) secretion were measured by ELISA. The data are mean ± S.D. of triplicate samples and is representative of three experiments. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared with EV-containing THP-1 cells. N.D., not detected.