Figure 6.
DHX9 is required for TLR2- and TLR8-stimulated IL-6 mRNA induction. A and B, THP-1 cells expressing doxycycline-inducible DHX9-specific shRNA (A7 and G2) or NSC shRNA were incubated with 1 μg/ml of doxycycline for 72 h, and then stimulated with 2 μg/ml of Pam3CSK4 (PAM3) or 2.5 μg/ml of CLO75 for the indicated times. IL-6 mRNA was assayed by quantitative PCR. Data are presented as relative expression of IL-6 normalized to β-actin compared with nonstimulated NSC shRNA-producing cells. The data are mean ± S.D. of triplicate samples and is representative of three experiments. ***, p < 0.001 compared with NSC.