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DHX9 is not required for TLR2-stimulated NF-κB or p38 MAPK activation. THP-1 cells expressing doxycycline-inducible DHX9-specific shRNA (G2) or NSC shRNA, were incubated with 1 μg/ml of doxycycline for 72 h, and then stimulated with 2 μg/ml of Pam3CSK4 (PAM3) for the indicated times. Cell lysates were harvested and immunoblotted for DHX9, IκB, β-actin, phospho-p65, total p65, phospho-p38, and total p38 as indicated. These data are representative of three experiments.
