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. 2018 Aug 13;293(39):15136–15151. doi: 10.1074/jbc.RA118.003290

Figure 7.

Figure 7.

Key differences in ACK and WASP binding to Cdc42. A, alignment of the ACK GBD and WASP BR–GBD sequences. The UniProt accession number for WASP is P42768 and for ACK is Q07912. Residues are colored according to their effect on equilibrium constant: gray, 0–5-fold increase; green, 5–10-fold increase; blue, 10–50-fold increase; orange, 50–100-fold increase; red, 100 or greater fold increase. The CRIB region, N- and C-terminal BR triads, and N- and C-terminal regions of the GBDs are labeled. B, schematic representation of part of the Cdc42–WASP GBD (yellow/magenta, PDB code 1CEE (14)) and Cdc42–ACK GBD (green/cyan, respectively, PDB code 1CF4 (12)) structures highlighting Pro-241WASP and Pro-457ACK. C, schematic representation of part of the Cdc42–WASP GBD (yellow/magenta, PDB code 1CEE (14)) and Cdc42–ACK GBD (green/cyan, respectively, PDB code 1CF4 (12)) structures highlighting the positions of the C-terminal regions of the WASP and ACK GBDs. Coloring is as in B. The nucleotide is shown as a stick representation, colored orange.