Table 1.
WASP residues mutated in this study
| WASP residue | Location | Interaction partner(s) on Cdc42 |
|---|---|---|
| Lys-225 | Basic region | a |
| Lys-226 | Basic region | a |
| Arg-227 | Basic region | a |
| Lys-230 | Basic region | a |
| Lys-231 | Basic region | a |
| Lys-232 | Basic region | a |
| Ile-233 | Cdc42/WASP structure | Ile-46, Leu-174, Leu-177 |
| Lys-235 | Basic region | Lys-153, Glu-171, Leu-174 |
| Ile-238 | CRIB residue | Val-44, Ile-46, Asp-170, Ile-173, Leu-174 |
| Pro-241 | CRIB residue | Tyr-23, Val-42, Val-44, Lys-166 |
| Ser-242 | Cdc42/WASP structure | Val-42, Thr-43 |
| Phe-244 | CRIB residue | Thr-24, Thr-25, Tyr-40, Val-42 |
| His-246 | CRIB residue | Tyr-40 |
| His-249 | CRIB residue | Asp-38 |
| Val-250 | CRIB residue | Val-36, Phe-37 |
| Gly-251 | CRIB residue | b |
| Trp-252 | Cdc42/WASP structure | Val-36 |
| Leu-267 | Cdc42/WASP structure | Phe-37, Leu-70 |
| Leu-270 | Cdc42/WASP structure | Leu-70 |
| Phe-271 | Cdc42/WASP structure | Phe-37, Leu-67, Leu-70 |
a Residues of the basic region do not appear to bind to specific residues on Cdc42 but rather make nonspecific interactions with negatively charged residues in the vicinity.
b Gly-251 is a CRIB residue that does not appear to interact specifically with any Cdc42 residues in the Cdc42/WASP structure.