Table 2.
Mutation | Kda | -Fold increase | ΔG | ΔΔG |
---|---|---|---|---|
nm | kcal/mol | cal/mol | ||
WT | 0.5 ± 0.1 | −12.4 | ||
I233A | 26.4 ± 2.4 | 52.8 | −10.1 | 2.3 |
K235A | 8.0 ± 0.6 | 16.0 | −10.8 | 1.6 |
I238A | NDb | >2000c | ||
P241A | 38.3 ± 3.0 | 76.6 | −9.9 | 2.5 |
S242A | 6.4 ± 0.9 | 12.8 | −10.9 | 1.5 |
F244A | 64.9 ± 7.7 | 129.8 | −9.6 | 2.8 |
H246A | 42.3 ± 4.5 | 84.6 | −9.8 | 2.6 |
H249A | 43.1 ± 2.3 | 86.2 | −9.8 | 2.6 |
V250A | 10.1 ± 1.1 | 20.2 | −10.7 | 1.7 |
G251A | 5.2 ± 0.5 | 10.4 | −11.0 | 1.4 |
W252A | 9.6 ± 0.9 | 19.2 | −10.7 | 1.7 |
L267A | 3.2 ± 0.5 | 6.4 | −11.3 | 1.1 |
L270A | 1.7 ± 0.4 | 3.4 | −11.7 | 0.7 |
F271A | 5.0 ± 0.8 | 10.0 | −11.1 | 1.3 |
201–254 | 31.8 ± 2.7 | 63.6 | −10.0 | 2.4 |
a The standard error from curve fitting is shown.
b ND (no binding) denotes data that could not be fitted to the binding isotherm.
c We assume a Kd of >1 μm (the limit of accurate direct SPA calculations).