Table 3.

Equilibrium binding constants and thermodynamic data for WASP BR mutants

Mutation	Kda	-Fold increase	ΔG	ΔΔG
	μm		kcal/mol	cal/mol
WT	0.5 ± 0.1		−12.4
K225A	2.8 ± 0.3	5.6	−11.4	1.0
K226A	5.4 ± 0.8	10.8	−11.0	1.4
R227A	4.3 ± 0.4	8.6	−11.1	1.2
K225A/K226A	7.4 ± 1.2	14.8	−10.8	1.6
K225A/R227A	7.5 ± 1.0	15	−10.8	1.6
K226A/R227A	5.7 ± 0.7	11.4	−11.0	1.4
K225A/K226A/R227A	8.8 ± 1.0	17.6	−10.7	1.7
K230A	9.7 ± 0.7	19.4	−10.7	1.7
K231A	12.4 ± 1.0	24.8	−10.5	1.9
K232A	12.2 ± 1.0	24.4	−10.5	1.8
K230A/K231A	12.9 ± 1.2	25.8	−10.5	1.9
K230A/K232A	18.4 ± 2.1	36.8	−10.3	2.1
K231A/K232A	21.7 ± 2.8	43.4	−10.2	2.2
K230A/K231A/K232A	36.0 ± 3.1	72	−9.9	2.5
K225A/K226A/R227A/K230A/K231A/K232A	98.6 ± 10.9	197	−9.3	3.1

^a The standard error from curve fitting is shown.