Table 4.

Binding kinetics for WASP variants with Cdc42

Mutation	kona	koff	Kd
	μm−1 s−1	s−1	nm
WT	0.691 ± 0.096	0.0417 ± 0.0113	63.7 ± 26.2
K225A	0.671 ± 0.215	0.0468 ± 0.0168	83.5 ± 45.2
K226A	0.676 ± 0.136	0.0338 ± 0.0106	54.4 ± 24.7
R227A	0.630 ± 0.144	0.0281 ± 0.0086	49.0 ± 23.2
K225A/K226A	0.490 ± 0.019	0.0295 ± 0.0073	59.6 ± 12.4
K225A/R227A	0.456 ± 0.017	0.0371 ± 0.0040	81.5 ± 8.7
K226A/R227A	0.497 ± 0.018	0.0372 ± 0.0004	74.8 ± 2.8
K225A/K226A/R227A	0.540 ± 0.065	0.0420 ± 0.0014	79.2 ± 12.2
K230A	0.477 ± 0.042	0.0498 ± 0.0029	105.5 ± 12.2
K231A	0.509 ± 0.082	0.0438 ± 0.0052	89.6 ± 24.5
K232A	0.407 ± 0.020	0.0546 ± 0.0008	134.0 ± 6.8
K230A/K231A	0.422 ± 0.014	0.0684 ± 0.0028	162.5 ± 12.0
K230A/K232A	0.484 ± 0.077	0.0670 ± 0.0026	141.8 ± 23.3
K231A/K232A	0.368 ± 0.004	0.0639 ± 0.0001	177.7 ± 0.5
K230A/K231A/K232A	0.300 ± 0.039	0.0914 ± 0.0096	307.4 ± 37.3
K225A/K226A/R227A/K230A/K231A/K232A	0.198 ± 0.019	0.1450 ± 0.0034	734.0 ± 72.6

^a The mean values for k_on, k_off, and K_d with standard errors from three independent experiments are shown.