Figure 1.

Ubiquitination reactions catalyzed by NopM and variants. A, Western blot (WB) analysis of ubiquitination reactions with NopM and the variants C338A (no activity) and NEL (increased activity). Recombinant E1, E2, and Ub proteins of A. thaliana were used for the reactions. Western blottings were developed with anti-ubiquitin (top) or anti-NopM (bottom) antibodies. B and C, ubiquitination reactions catalyzed by NopM or NEL with ubiquitin or indicated ubiquitin variants with lysine to arginine substitutions. Control reactions without ubiquitin are also shown (lanes C). Western blot analysis was performed with anti-ubiquitin (B) or anti-NopM (C) antibodies. Lane M, molecular weight markers.