Figure 1.

Characterization of IRAK4 variant patient dermal fibroblasts. SV40-transformed human dermal fibroblasts were stimulated with 10 ng/ml IL-1β and assayed for phosphoprotein expression and cytokines (WT homozygote (WT); IRAK4-null homozygote (P15), and R12C.R391H.T458I/IRAK4-null heterozygote (R12C)). A, immunoblots of whole-cell lysates at the indicated times of IL-1β stimulation. The blot is representative of three independent experiments. B, quantitation of IRAK4 expression as determined by immunoblot in A and normalized to tubulin. C, measurement of secreted IL-8 following 4 h of IL-1β treatment. Data reflect the mean of three independent experiments, significance determined by one-way analysis of variance. NS, not significant. Error bars, S.D.