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Expression and kinase activity of variants of IRAK4. A, Western blotting of cell lysates from COS7 cells overexpressing IRAK4 constructs. B, Western blotting of FLAG immunoprecipitated autophosphorylated IRAK4 constructs expressed in COS7 cells. C, kinase activity of purified IRAK4 constructs expressed in COS7 cells normalized to IRAK4 protein expression. Data reflect the mean of three independent experiments. IP, immunoprecipitation. Error bars, S.E.
