Figure 6.

Inhibition of IL-1–induced signaling and cytokine in primary dermal fibroblasts using an IRAK4 inhibitor. A, primary human neonatal dermal fibroblasts in the presence of either DMSO or 200 nm IRAK4 inhibitor PF-06650833 were stimulated with 2 ng/ml IL-1β overnight, after which IL-6 and IL-8 secretion was measured. B, IL-6 and IL-8 transcripts as determined following stimulation with 2 ng/ml IL-1β for 6 h in the presence of either DMSO or 200 nm IRAK4 inhibitor PF-06650833. C, primary human neonatal dermal fibroblasts were stimulated with 2 ng/ml IL-1β for the indicated times in the presence of DMSO or 200 nm PF-06650833 and lysed and immunoblotted for the indicated proteins. D, quantitation of phosphoproteins from C relative to parent protein (DMSO (black) or PF-06650833 (red)). Data represent the mean of three independent experiments.