Figure 7.

Immunoprecipitation of IRAK4-associated proteins from primary human dermal fibroblasts in the presence of the selective IRAK4 inhibitor PF-06650833. A, primary human neonatal dermal fibroblasts stimulated with 2 ng/ml IL-1β for the indicated time in the presence or absence of 200 nm PF-06650833 were lysed, and IRAK4 was immunoprecipitated and immunoblotted for the indicated proteins. The image represents one example of four independent experiments. B, quantitation of proteins from A relative to total IRAK4 (DMSO (black) or PF-06650833 (red)). Data represent the mean of four independent experiments. Error bars, S.D.