Figure 3.

Level of protein expression for TSP-1, CD47, and CD36 in osteoblasts and osteoclasts. A, representative Western blot for TSP-1, CD47, and CD36 in whole-cell lysates prepared from primary murine osteoclasts at the indicated days of differentiation induced with RANKL and CSF-1. During osteoclast differentiation, TSP-1 protein is expressed at Day 0 and is not expressed at Day 2 or Day 4. CD47 is not expressed at Day 0 but is seen at Day 2 and Day 4. CD36 is only expressed at Day 0. B, osteoblasts express CD47 but do not express CD36 or TSP-1. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.