Table 1.
Substrate specificities of human GlcNAc6STs
| Enzyme | HGNCa | Alternative names | Predominant tissue distribution | Preferred substrates (in vitro) | Reference(s) |
|---|---|---|---|---|---|
| GlcNAc6ST-1 | CHST2 | Ubiquitously expressed | GlcNAcβ2/6ManOMe, GlcNAcβ6 (Galβ3)GalNAc-pNP, not core 3–based structure | 18 | |
| GlcNAc6ST-2 | CHST4 | HEC-GlcNAc6ST, LSST | HEC cells | GlcNAcβ2/6ManOMe, GlcNAcβ6(Galβ3)GalNAc-pNP, GlcNAcβ3GalNAc-pNP | 17, 18, 23 |
| GlcNAc6ST-3 | CHST5 | I-GlcNAc6ST | Colon, intestine | GlcNAcβ6(Galβ3)GalNAc-pNP, not core 3–based structure, GlcNAcβ2Manα6Manβ1-octyl, GlcNAcβ2(GlcNAcβ6)-Manα6Manβ1-octyl | 17, 18, 24 |
| GlcNAc6ST-4 | CHST7 | C6ST-2 | Ubiquitously expressed | GlcNAcβ2/6ManOMe, GlcNAcβ6(Galβ3)GalNAc-pNP, GlcNAcβ3GalNAc-pNP | 19 |
| GlcNAc6ST-5 | CHST6 | C-GlcNAc6ST | Cornea | GlcNAcβ6Manα6Manβ1-octyl, GlcNAcβ3Galβ4GlcNAcβ6-Manα6Manβ1-octyl, GlcNAcβ3Galβ4GlcNAcβ3Galβ4-GlcNAcβ6Manβ6Manβ1-octyl, GlcNAcβ6(Galβ3)GalNAc-octyl | 22, 24 |
a HGNC (HUGO Gene Nomenclature Committee)-approved gene names; CHST, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase.