Figure 3.

Upregulation of p-PKCβII in the DRG after SMIR. (A) Expression of p-PKCβII 7 days after SMIR was determined by densitometric analysis on Western blots using PKCβII as control. Compared with the untreated control and Sham groups, expression levels of p-PKCβII were significantly higher 7 days after SMIR (*P < 0.05); n=5 for each group. (B) Average values (SD) of densitometric analysis of p-PKCβII-positive cells in the DRG in SMIR, naïve, and sham groups. SMIR and Sham values were compared for the same time and segment by one-way analysis of variance (***P < 0.001); n=5 for each group. (C) Immunostaining pictures of the p-PKCβII expression in the DRG in naïve, sham, and SMIR animals.

Abbreviations: SMIR, skin/muscle incision and retraction; DRG, dorsal root ganglion; PKCβII, protein kinase C βII.