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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Nature. 2018 Sep 12;561(7724):492–497. doi: 10.1038/s41586-018-0535-y

Extended Data Figure 2. CGRP receptor pharmacology and purification of the CGRP-CLR-RAMP1-Gs complex.

Extended Data Figure 2

A; Pharmacology of untagged CLR-RAMP1 (WT CLR-RAMP1) and the purification construct (HA–FLAG-CLR, FLAG-RAMP1), in CGRP-mediated cAMP accumulation assays performed in transiently transfected Cos7 cells (N=5 separate experiments with triplicate repeats; mean + s.e.). B; Expression and purification strategy. C; Final size exclusion chromatography (SEC) elution profile of the complex. D; SDS–PAGE/Coomassie blue stain of the SEC peak, demonstrating presence of each of the components of the complex.