Extended Data Figure 4. Atomic resolution model of the CGRP–CLR–RAMP1-Gs heterotrimer in the cryo-EM density map.

EM density map and model are shown for all seven transmembrane helices and H8 of the receptor, the CGRP peptide (excepting the K24^PA25^PN26^P sequence that was not resolved in the map), the RAMP TM and each of the RAMP ECD helices; there was only limited side chain density for RAMP1 H1, with side chains modeled from rigid body fitting of the RAMP1 ECD in PDB: 4RWG¹². Also illustrated are the N-terminal (αH1) and C-terminal (αH5) α-helices of the Gαs-Ras domain.