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. 2018 Oct 1;13(10):e0204853. doi: 10.1371/journal.pone.0204853

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© 2018 Wong et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A, B) HEK293T cells were transfected with HA-tagged NRF2 and either wild type FLAG-tagged KEAP1 or C151S KEAP1 mutant for 48 hours before treatment with andrographolide (7.5 μM), arsenite (20 μM), PMX290 (2 μM), or sulforaphane (10 μM) for 6 hours. The cell lysate samples were subjected to SDS-PAGE and analyzed using Western Blotting with HA and FLAG antibodies.