Fig 3. Effect of andrographolide on the interaction between KEAP1 and CUL3.

(A, B) The cells were transfected with the indicated expression plasmids for two days, followed by drug treatment with 7.5 μM andrographolide (A) or 2 μM PMX290 (B) for 4 hours or 6 hours, respectively. Cells were then lysed and subjected to FLAG immunoprecipitation, as described under Materials and Methods. The data in (C) represent the mean and S.E.M. of 5 independent repeats of the immunoprecipitation in (A).