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. 2018 Oct 1;13(10):e0204924. doi: 10.1371/journal.pone.0204924

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© 2018 Sobhkhez et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Expression of type I IFN inducible genes (A), IFNγ inducible genes (B), and type I IFN (IFNa) and type II IFN (IFNγ) genes (C) in response to vaccination with plasmids. Groups of fish (N = 5) were injected with PBS, pcDNA3.3 and pSAV. RNA was isolated from muscle at the injection site 7 days post injection and expression of different IFNs and ISGs was measured by RT-qPCR. Expression value of each gene was normalized against the levels of EF1αβ. Numbers represents fold increase in expression (mean ± SD) compared to the PBS group. Bars not sharing common letter indicate significant difference (p ≤ 0.05).