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. 2018 Oct 1;13(10):e0204860. doi: 10.1371/journal.pone.0204860

Fig 4. Expression of C/ebpβ in the injury model and in the mmu-miR-155- overexpressing cells, and effect of C/ebpβ expression on myogenic differentiation in C2C12 cells.

Fig 4

(A) expression level of C/ebpβ mRNA in the injury model. (B) expression level of C/ebpβ mRNA in the mmu-miR-155 overexpressing C2C12 cells (N = 3). (C) expression level of C/ebpβ mRNA in the cumate-treated C2C12 cells containing the QM-mmu-miR-155 system (right). Expression of exogenous miR-155 was induced by cumate addition (N = 3). Asterisks indicate significant differences compared to the sham control. (D) effect of C/ebpβ overexpression on the differentiation of C2C12 cells. Samples were collected at day 2 after differentiation induction (N = 3). Asterisks indicate significant differences at P < 0.05 compared to the control cells containing an empty plasmid. (E) WB analysis for the differentiated markers MyoD, Myogenin, and Mrf4 in the C/ebpβ overexpressing C2C12 cells at day 2 of differentiation induction.