Figure 5. FIPs inhibit adipocyte differentiation from APCs.

(A) Pparg isoform two expression in cultured APCs maintained for 3 days in conditioned media from either APCs, FIPs, or MLCs. n = 4. *denotes p<0.05 by student’s t-test in comparisons to data represented in blue bars. Bars represent mean +SEM. (B) Brightfield image of APCs after 8 days of culture in conditioned media from parallel cultures of APCs. Scale bar = 100 μm for B-D. (C) Brightfield image of APCs after 8 days of culture in conditioned media from parallel cultures of FIPs. (D) Brightfield image of APCs after 8 days of culture in conditioned media from parallel cultures of MLCs. (E) mRNA levels of adipocyte-selective genes within cultures shown in (B–D). n = 3. * denotes p<0.05 by student’s t-test in comparisons to data represented in blue bars. Bars represent mean +SEM. (F) Distribution of Abcg1 and F3 expression within tSNE plot from Figure 1B. (G) mRNA levels of Abcg1 and F3 in APCs, FIPs, and MLCs isolated from lean 8 week old male mice. * denotes p<0.05 by student’s t-test. Bars represent mean +SEM. (H) tSNE-plot highlighting the potential relationship between APCs, FIPs, and MLCs, and iguinal WAT Aregs identified by Schwalie et al. (Schwalie et al., 2018). The top-23 Areg-selective genes identified by Schwalie et al were input into Cell Loupe Browser. Color intensities represent the sum of the Log₂ expression values of the Areg selective gene list within the single cell RNA-sequencing dataset of gWAT from Figure 1B.