Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-guinea pig Alexa 647 | Invitrogen | RRID:AB_141882 | 1:200 |
| Antibody | anti-chicken Alexa 488 | Invitrogen | RRID:AB_142924 | 1:200 |
| Antibody | anti-GFP | Abcam | RRID:AB_300798 | 1:700 |
| Antibody | anti-Perilipin | Fitzgerald | RRID:AB_1288416 | 1:1500 |
| Antibody | CD24-APC | eBioscience | RRID:AB_10852841 | 1:400 |
| Antibody | CD31-FITC | Biolegend | RRID:AB_312900 | 1:400 |
| Antibody | CD31-PerCP/Cy5.5 | Biolegend | RRID:AB_10612742 | 1:400 |
| Antibody | CD34-APC | Biolegend | RRID:AB_10553895 | 1:400 |
| Antibody | CD38-FITC | Biolegend | RRID:AB_312926 | 1:400 |
| Antibody | CD45-FITC | Biolegend | RRID:AB_312973 | 1:400 |
| Antibody | CD45-PerCP/Cy5.5 | Biolegend | RRID:AB_893344 | 1:400 |
| Antibody | CD9-APC | eBioscience | RRID:AB_10669565 | 1:400 |
| Antibody | CD9-FITC | Biolegend | RRID:AB_1279321 | 1:400 |
| Antibody | FC Block | BD Biosciences | RRID:AB_394657 | 1:200 |
| Antibody | LY6C-APC | Biolegend | RRID:AB_1732076 | 1:400 |
| Antibody | LY6C-BV421 | Biolegend | RRID:AB_2562178 | 1:400 |
| Antibody | PDGFRα-APC | Biolegend | RRID:AB_2043970 | 1:200 |
| Antibody | PDGFRβ-APC | Biolegend | RRID:AB_2268091 | 1:50 |
| Antibody | PDGFRβ-PE | Biolegend | RRID:AB_1953271 | 1:50 |
| Antibody | SCA-1-APC | Biolegend | RRID:AB_313348 | 1:400 |
| Chemical compound, drug | Trypsin | Corning | 25–052 Cl | |
| Chemical compound, drug | BrdU | Sigma | B5002 | |
| Chemical compound, drug | BSA | Fisher Scientific | BP1605 | |
| Chemical compound, drug | Collagenase D | Roche | 11088882001 | |
| Chemical compound, drug | Dexamethosone | Sigma | D4902 | |
| Chemical compound, drug | DMEM with 1 g/L glucose, L-glutamine, and sodium pyruvate |
Corning | 10–014-CV | |
| Chemical compound, drug | DMEM/F12 with GlutaMAX | Gibco | 10565–018 | |
| Chemical compound, drug | FBS | Sigma | 12303C | |
| Chemical compound, drug | FGF basic | R and D Systems | 3139-FB-025/CF | |
| Chemical compound, drug | Gentamicin Reagent (50 mg/ml) | Gibco | 15750–060 | |
| Chemical compound, drug | Harris Eosin Solution | Sigma | HT110116 | |
| Chemical compound, drug | Harris Hematoxylin Solution | Sigma | HHS16 | |
| Chemical compound, drug | HBSS | Sigma | H8264 | |
| Chemical compound, drug | Insulin | Sigma | I6634 | |
| Chemical compound, drug | Isobutylmethyxanthine | Sigma | I7018 | |
| Chemical compound, drug | ITS Premix | BD Bioscience | 354352 | |
| Chemical compound, drug | L-ascorbic acid-2-2phosphate | Sigma | A8960 | |
| Chemical compound, drug | Lipofectamine LTX | Invitrogen | 15338100 | |
| Chemical compound, drug | Lipopolysaccharides from Escherichia coli O111:B4 |
Sigma | L3024 | |
| Chemical compound, drug | Matrigel Growth Factor Reduced Membrane Matrix |
Corning | 354230 | |
| Chemical compound, drug | MCDB201 | Sigma | M6770 | |
| Chemical compound, drug | M-MLV RT | Invitrogen | 28025013 | |
| Chemical compound, drug | Oil Red O | Sigma | O0625 | |
| Chemical compound, drug | PBS | Sigma | D8537 | |
| Chemical compound, drug | pCMV-VSV-G | Addgene | 8454 | |
| Chemical compound, drug | Penicillin Streptomycin Solution | Corning | 30–001 Cl | |
| Chemical compound, drug | Polybrene | Sigma | TR-1003 | |
| Chemical compound, drug | psPAX2 | Addgene | 12260 | |
| Chemical compound, drug | Random Primers | Invitrogen | 48190011 | |
| Chemical compound, drug | Recombinant Human TGFβ−1 | R and D Systems | 240-B-002 | |
| Chemical compound, drug | Recombinant Murine TNFα | PeproTech | 315-01A | |
| Chemical compound, drug | Red Blood Cell Lysing Buffer Hybri-Max |
Sigma | R7757 | |
| Chemical compound, drug | SYBR Green PCR Master Mix | Applied Biosystems | 4309155 | |
| Chemical compound, drug | Trizol | Invitrogen | 15596018 | |
| Commercial assay or kit | Chromium i7 Multiplex Kit, 96 rxns |
10X Genomics | 120262 | |
| Commercial assay or kit | Chromium Single Cell 3' Library and Gel Bead Kit v2, 16 rxns |
10X Genomics | 120237 | |
| Commercial assay or kit | Chromium Single Cell A Chip Kit, 48 rxns |
10X Genomics | 120236 | |
| Commercial assay or kit | Dynabeads MyOne Silane | Thermo Fisher Scientific | 37002D | |
| Commercial assay or kit | FITC BrdU Flow Kit | BD Biosciences | 559619 | |
| Commercial assay or kit | RNAqueous-Micro Total RNA Isolation Kit |
Invitrogen | AM1931 | |
| Commercial assay or kit | SPRIselect | Beckman Coulter | B23317 | |
| Other | 100 µm cell strainer | Falcon | 352360 | |
| Other | 12-well plate | Corning | 356500 | |
| Other | 40 µm cell strainer | Falcon | 352340 | |
| Other | 48-well plate | Falcon | 353230 | |
| Other | doxycyline-containing chow diet (600 mg/kg doxycycline) |
Bio-Serv | S4107 | |
| Other | high-fat diet (60% kcal% fat) | Research Diets | D12492i | |
| Software, algorithm | Cell Ranger v2.1.0 | 10X Genomics | NA | |
| Software, algorithm | FlowJo V10 | FlowJo | RRID:SCR_008520 | |
| Software, algorithm | Gene Set Enrichment Analysis v3.0 |
Broad Institute | RRID:SCR_003199 | |
| Software, algorithm | Graphpad Prism 7 | Graphpad | RRID:SCR_002798 | |
| Software, algorithm | R Studio v3.3.2 | RStudio | RRID:SCR_000432 | |
| Software, algorithm | Readr v1.1.0 | NA | NA | |
| Software, algorithm | Seurat v2.1.0 | Satija Lab | RRID:SCR_016341 | |
| Strain, strain background (M. musculus, C57BL/6) |
C57BL/6 | Charles River Laboratories | RRID:IMSR_CRL:27 | |
| Strain, strain background (M. musculus, C57BL/6) |
PdgfrbrtTA | Jackson Laboratories | RRID:IMSR_JAX:028570 | |
| Strain, strain background (M. musculus, C57BL/6) |
Rosa26RmT/mG | Jackson Laboratories | RRID:IMSR_JAX:007676 | |
| Strain, strain background (M. musculus, C57BL/6) |
TRE-Cre | Jackson Laboratories | RRID:IMSR_JAX:006234 | |
| Strain, strain background (M. musculus, C57BL/6) |
Zfp423GFP | Other | Zfp423GFPB6 | PMID: 26626462 |