Skip to main content
. 2018 Sep 25;2(10):1247–1258. doi: 10.1002/hep4.1240

Figure 3.

Figure 3

CEACAM1‐mediated suppression of NK cell function required cell–cell contact. (A‐C) Huh7.5.1/JFH‐1 cells were cultured in the bottom of the 96‐well Transwell cell culture system, and NK cells were cultured on the membrane of the Transwell cell culture inserts or in the bottom of the plate. After 24 hours of coculture in a Transwell, CFSE‐labeled K562 cells were added to NK cells and incubated for 6 hours. We subsequently measured the degree of cytotoxicity toward K562 cells (A), CD107a expression on NK cells (B), and IFN‐γ expression in NK cells (C) using flow cytometry (means ± SD, n = 4). Representative data obtained from the peripheral NK cells of healthy subjects are shown. * P < 0.05.