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. 2018 Sep 29;33(3):301–308. doi: 10.1264/jsme2.ME17186

Fig. 3.

Fig. 3

Qualitative and quantitative assessments of H2O2 in B510-inoculated rice grown on RG medium.

DAB staining was performed 10 d after the inoculation of rice seeds with B510. (A) The accumulation of H2O2 was quantified in the roots using standard H2O2 concentrations to calibrate data during optical density (OD) measurements. Plants were grown in RG medium including 1 mM of KNO3, 1 mM NH4Cl, 1 mM NH4Cl+100 mM MES, or without nitrogen (control), inoculated with B510 (+) or not inoculated (−). Error bars indicate standard deviations (n=3). (B) H2O2 levels correlate with color intensity (brown). The arrow indicates root curling. Scale bar=0.5 cm.