Table 1.
Properties of newly designed bacterial primers, which were designed by improving the original primer set of modified 63f and 1492r
| Primer | 5′-Sequence-3′ | Tm value | Coverage | Size | Position in E. coli | References |
|---|---|---|---|---|---|---|
| Forward Primer | ||||||
| modified 63f | YRKGCYTWAYACATGCAAGTC | 58–67°C | 76.3% | 21mer | 43–63 | Ikenaga and Sakai (13) |
| KU63f | GCYTWAYACATGCAAGTC | 54–60°C | 80.6% | 18mer | 46–63 | This study |
| KU64f | GCYTWAYACATGCAAGTCG | 57–62°C | 80.0% | 19mer | 46–64 | This study |
| KU68f | AYACATGCAAGTCGARCG | 58–64°C | 73.0% | 18mer | 51–68 | This study |
|
| ||||||
| Reverse Primer | ||||||
| 1492r | GGYTACCTTGTTACGACTT | 57–60°C | 81.2% | 19mer | 1510–1492 | Ikenaga and Sakai (13) |
| KU1494r | GGYTACCTTGTTACGAC | 55–58°C | 81.7% | 17mer | 1510–1494 | This study |
Y=C or T; R=A or G; K=T or G; W=A or T