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Positions of complementary sequences at the 3′ end between forward and reverse primers
| Primer | Sequence | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| modified 63f | 5′ | Y | R | K | G | C | Y | T | W | A | Y | A | C | A | T | G | C | A | A | G | T | C | – | 3′ | |||||||||||||
| KU63f | 5′ | G | C | Y | T | W | A | Y | A | C | A | T | G | C | A | A | G | T | C | – | 3′ | ||||||||||||||||
| KU64f | 5′ | G | C | Y | T | W | A | Y | A | C | A | T | G | C | A | A | G | T | C | G | 3′ | ||||||||||||||||
| KU68f | 5′ | A | Y | A | C | A | T | G | C | A | A | G | T | C | G | A | R | C | G | 3′ | |||||||||||||||||
|
| |||||||||||||||||||||||||||||||||||||
| 1492r | 3′ | T | T | C | A | G | C | A | T | T | G | T | T | C | C | A | T | Y | G | G | 5′ | ||||||||||||||||
| KU1494r | 3′ | – | – | C | A | G | C | A | T | T | G | T | T | C | C | A | T | Y | G | G | 5′ | ||||||||||||||||
“
” indicates the positions showing complimentary sequences.
